Coding

Part:BBa_K5048052

Designed by: Yiheng Liao   Group: iGEM24_WHU-China   (2024-09-22)


lasR-dt-plas-ccdB-dt

This fragment will work together with the araBAD promoter on the pBAD backbone. Upon induction with arabinose, the bacteria will express lasR. On this basis, with the addition of lasI, the bacteria will express the ccdB toxin and die.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 322
    Illegal AgeI site found at 519
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1194


[edit]
Categories
Parameters
None